Search results for "Xyloglucan:xyloglucosyl transferase"
showing 3 items of 3 documents
Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls.
2013
Abstract: Growth and biomechanics of etiolated hypocotyls from Arabidopsis thaliana lines overexpressing xyloglucan endotransglucosylase/hydrolase AtXTH18, AtXTH19, AtXTH20, and PttXET16-34 were studied. Overexpression of AtXTH18, AtXTH19, and AtXTH20 stimulated growth of hypocotyls, while PttXET16-34 overexpression did not show this effect. In vitro extension of frozen/thawed hypocotyls measured by a constant-load extensiometer started from a high-amplitude initial deformation followed by a slow time-dependent creep. Creep of growing XTH-overexpressing (OE) hypocotyls was more linear in time compared with the wild type at pH 5.0, reflecting their higher potential for long-term extension. X…
Xyloglucan endotransglucosylase and cell wall extensibility
2011
Transgenic tomato hypocotyls with altered levels of an XTH gene were used to study how XET activity could affect the hypocotyl growth and cell wall extensibility. Transgenic hypocotyls showed significant over-expression (line 13) or co-suppression (line 33) of the SlXTH1 in comparison with the wild type, with these results being correlated with the results on specific soluble XET activity, suggesting that SlXTH1 translates mainly for a soluble XET isoenzyme. A relationship between XET activity and cell wall extensibility was found, and the highest total extensibility was located in the apical hypocotyl segment of the over-expressing SlXTH1 line, where the XET-specific activity and hypocotyl…
Cell wall xyloglucan incorporation by xyloglucan endotransglucosylase/hydrolase in pine hypocotyls
2004
Abstract In the present study, we have examined the incorporation of labelled xyloglucan oligosaccharides into the cell wall xyloglucan of pine hypocotyl sections, and the effect of plant hormones on the incorporation and on XTH enzyme activity (EC 2.4.1.207). Xyloglucan heptasaccharide [ 3 H ]XXXGol was absorbed, transported and incorporated into the cell wall xyloglucan of de-rooted pine hypocotyls. Incorporation of [ 3 H ]XXXGol into soluble and cell wall xyloglucan was induced by IAA, brassinolides and XXXG in pine hypocotyl segments. A relationship between growth induction, by IAA, brassinolides and XXXG, and both soluble and cell wall-bound XTH was found. We suggest that IAA induced-g…